Collection of Blood Samples

1. A 3-5ml blood sample is drawn into a pre-chilled (4°C) EDTA(Na2) vacutainer tube (Becton-Dickson).
2. The sample is gently mixed 5-6 times by hand and maintained on ice.
3. Transfer 300µl aliquots into six 1.5ml cryogenic tubes (Sarstedt).
4. Immediately freeze at -80°C.
5. Be sure all tubes are labeled clearly.

Shipping Samples

1. Samples must be shipped in dry ice by overnight carrier.
2. Work quickly to prevent the small volume of sample from thawing.
3. Foil wrap tubes to protect from light.
4. Place in plastic bags; then pack with dry ice.
5. Include a legible list of all samples being sent.
6. Call the lab in advance to speak to lab personnel to inform them of samples being shipped. DO NOT LEAVE VOICE MAIL OR EMAIL.
7. Ship to:

   Thomas Jefferson University Hospital
   Department of Anesthesiology Research Laboratory
   c/o Joanne Vesci
   1015 Walnut Street
   Curtis Building, Room 405
   Philadelphia, PA 19107

8. To notify lab or for additional information call: 215-955-6645.